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FISH Probe

A FISH (Fluorescence In Situ Hybridization) probe is a fluorescently labeled DNA or RNA sequence designed to bind to a specific region of genetic material within cells or tissues. When applied to a sample, the probe hybridizes to its complementary sequence, and the fluorescent signal can be visualized under a fluorescence microscope. This technique enables direct detection and localization of genes or chromosomes within intact cells.

FISH probes are widely used in both clinical diagnostics and research. They can help detect genetic abnormalities such as chromosomal translocations, deletions, duplications, and gene amplifications. For example, FISH is commonly used to assess HER2 gene status in breast cancer, identify chromosomal disorders like Down syndrome (trisomy 21), and detect gene fusions in leukemia. In research, FISH helps study gene expression, chromosome structure, and genome organization at the single-cell level.

Detailed FISH Probe Protocol can be found here: FISH-probe-procedure-Adherent-Cells.pdf

Human SERPINA1 FISH probe kit

Product Code: TDP-1017K-25
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Available Options

Size:
25ul (for 25 tests)
The human SERPINA1, labeled with ATTO565 dye, is designed to specifically hybridize to the SERPINA1 mRNA and gene locus in human cells and tissues. The SERPINA1 gene encodes alpha-1 antitrypsin (AAT), a major plasma serine protease inhibitor that plays a crucial role in protecting tissues from enzymes of inflammatory cells, especially neutrophil elastase. The kit contains all the necessary reagents and other materials to allow you to perform in situ hybridization with human SPERINA1 FISH probe for up to 25 tests
Chemical
Concentration 100ug/mL, The concentration of this product may be batch-dependent
Fluorophore Label Dye ATTO-565 with Excitation maximum: 565 nm and Emission maximum: ~580 nm
Biological
Entrez Gene ID 5265/NM_001295507
Gene Name SERPINA1/A1AT, AAT, A1PI
Number of Probe Pairs 31
Probe Type DNA Oligonucletide In Situ Hybridization (ISH) Assay Kit
Species Human
Target Region 1-1,532
Shipping and Handling
Shipped Product Format Liquid/Blue Ice
Storage Buffer TE (10 mM Tris-HCl,1 mM EDTA, pH 8.0)
Storage Condition 2-8 °C for short term, -20 or -80°C for long term; avoid freeze / thaw cycle
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