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6His-Cysteine
The HHHHHH-Cys peptide (6His) consists of six histidine residues followed by a cysteine residue and is used as a fusion tag for immunoaffinity chromatography. Polyhistidine tags, or 6xHis-tags, are commonly used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Chemical | |
---|---|
Form | Lyophilized |
Molecular Formula | C39H49N19O8S |
Molecular Weight | 944.01 |
Purity | >95% by HPLC |
Reconstitution | Reconstitute with PBS or H20 to desired concentration. Due to the nature of this peptide, it is recommended to first mix with 10 ul of DMSO. |
Sequence | HHHHHHC-NH2 |
Shipping and Handling | |
Storage Condition | Store at -20°C |