Gastric cancer cells are malignant cells originating from the lining of the stomach, representing a diverse group of cells that can vary significantly in morphology, molecular characteristics, and behavior. These cells are known to possess unique properties, such as rapid proliferation, evasion of apoptosis, and the ability to invade surrounding tissues and metastasize to distant organs. Research on gastric cancer cells has provided critical insights into the pathways driving gastric tumorigenesis, including alterations in signaling pathways like Wnt, Notch, and Hedgehog, and the impact of factors like Helicobacter pylori infection and chronic inflammation. Understanding the biology of gastric cancer cells is essential for developing targeted therapies and improving the prognosis of this challenging and often aggressive cancer type. TridixBio Patient Derived Gastric Tumor Cells were isolated from gastric cancer tissues obtained from patients undergoing surgical gastrectomy, and gastric cancer cells were identified using GRN and CA724 markers.