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FISH Probe

A FISH (Fluorescence In Situ Hybridization) probe is a fluorescently labeled DNA or RNA sequence designed to bind to a specific region of genetic material within cells or tissues. When applied to a sample, the probe hybridizes to its complementary sequence, and the fluorescent signal can be visualized under a fluorescence microscope. This technique enables direct detection and localization of genes or chromosomes within intact cells.

FISH probes are widely used in both clinical diagnostics and research. They can help detect genetic abnormalities such as chromosomal translocations, deletions, duplications, and gene amplifications. For example, FISH is commonly used to assess HER2 gene status in breast cancer, identify chromosomal disorders like Down syndrome (trisomy 21), and detect gene fusions in leukemia. In research, FISH helps study gene expression, chromosome structure, and genome organization at the single-cell level.

Detailed FISH Probe Protocol can be found here: FISH-probe-procedure-Adherent-Cells.pdf

Human Polr2a FISH probe kit

Product Code: TDP-1002K-25

Price: $249.00

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The human Polr2a FISH probe, labeled with ATTO-647N dye, is a molecular tool used to detect and visualize the Polr2a RNA and gene locus in human tissues or cells. This probe is labeled with ATTO-647N dyes that specifically bind to the mRNA of the Polr2a gene, allowing for the observation of its expression and localization within cells or tissue sections. The kit contains all the necessary reagents and other materials to allow you to perform in situ hybridization with human Polr2a FISH probe for up to 25 tests
Chemical
Concentration 100ug/mL, The concentration of this product may be batch-dependent
Fluorophore Label Dye ATTO-647N with Excitation maximum: ~650 nm and Emission maximum: ~668 nm
Biological
Entrez Gene ID 5430/NM_000937.5
Gene Name RNA polymerase II subunit A/RPB1, POLR2, POLRA
Number of Probe Pairs 48
Probe Type DNA Oligonucletide In Situ Hybridization (ISH) Assay Kit
Species Human
Target Region 1-5,913
Shipping and Handling
Shipped Product Format Liquid/Blue Ice
Storage Buffer TE (10 mM Tris-HCl,1 mM EDTA, pH 8.0)
Storage Condition 2-8 °C for short term, -20 or -80°C for long term; avoid freeze / thaw cycle
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