**Each order includes 2 vials**

The HEK293 GCGR β-arrestin cell line developed at TriDix Bio is a clonal HEK293 derivative engineered to stably express GCGR fused to a small luciferase subunit (SmBiT) and β-arrestin fused to a large luciferase subunit (LgBiT). Upon Glucagan ligand engagement, receptor activation drives β-arrestin recruitment, bringing SmBiT and LgBiT into proximity to reconstitute an active luciferase enzyme. In the presence of a substrate such as Furimazine, this interaction generates a robust luminescent signal, allowing reproducible quantification of ligand-dependent arrestin recruitment in live cells. The stable line is optimized for consistent expression, broad signal window, and compatibility with high-throughput, plate-based screening.

HEK293 GCGR β-arrestin cell line (TDC-S1502) Product Sheet