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Organoid Generation and Expansion
At TriDix, organoid generation is one of our core strengths. We specialize in generating organoids from primary mouse and human tissues and also provide the necessary media and Matrigel for these cultures. All our organoid lines undergo rigorous quality control, including mycoplasma testing, species identification, STR analysis and expressing profiling. For new organoid models without an established protocol at TriDix, we collaborate closely with our clients, either adapting the client’s protocol or drawing from existing published protocols.
Additionally, we can assist in scaling up organoids from your own lines, offering ready-to-use organoids that save you the time and effort of culturing them. Upon request, we can also provide customized quality control options tailored to your specific needs.
Spheroid/Organoid Culture Workflow

Step 1
Primary cell isolation from tissue

Step 2
Development of 3D culture

Step 3
Organoid culture

Step 4
Monitoring and reading

Step 5
Imaging, assay and other applications
Case Study: Generation of Liver Spheroids
Liver spheroids generated in 96-well plates: optimized disease modeling, low-tomedium-throughput testing, imaging-based analysis, and other research applications.
Liver spheroids generated in Honeycomb Culture Chip: enabling the convenient, high-throughput production of large quantities of spheroids in a single batch, ideal for downstream applications such as proteomic analysis