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FISH Probe

A FISH (Fluorescence In Situ Hybridization) probe is a fluorescently labeled DNA or RNA sequence designed to bind to a specific region of genetic material within cells or tissues. When applied to a sample, the probe hybridizes to its complementary sequence, and the fluorescent signal can be visualized under a fluorescence microscope. This technique enables direct detection and localization of genes or chromosomes within intact cells.

FISH probes are widely used in both clinical diagnostics and research. They can help detect genetic abnormalities such as chromosomal translocations, deletions, duplications, and gene amplifications. For example, FISH is commonly used to assess HER2 gene status in breast cancer, identify chromosomal disorders like Down syndrome (trisomy 21), and detect gene fusions in leukemia. In research, FISH helps study gene expression, chromosome structure, and genome organization at the single-cell level.

Detailed FISH Probe Protocol can be found here: FISH-probe-procedure-Adherent-Cells.pdf

Mouse Actb FISH probe kit

Product Code: TDP-7005K-25
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Available Options

Size:
25ul (for 25 tests)
Mouse Actb FISH probe, labeled with ATTO-565 is specifically designed for the in situ detection of ACTB mRNA and gene locus in mouse tissue or cell samples. This high-performance, fluorescently labeled probe ensures sensitive and specific hybridization, allowing for clear and precise visualization of ACTB expression through FISH. The kit contains all the necessary reagents and other materials to allow you to perform in situ hybridization with mouse Actb FISH probe for up to 25 tests
Chemical
Concentration 100ug/mL, The concentration of this product may be batch-dependent
Fluorophore Label Dye ATTO-565 with Excitation maximum: 565 nm and Emission maximum: ~580 nm
Biological
Entrez Gene ID 11461/NM_007393.5
Gene Name β-ACTIN/Actx, E430023M04Rik, ACTB
Number of Probe Pairs 25
Probe Type DNA Oligonucletide In Situ Hybridization (ISH) Assay Kit
Species Mouse
Target Region 1-10,128
Shipping and Handling
Shipped Product Format Liquid/Blue Ice
Storage Buffer TE (10 mM Tris-HCl,1 mM EDTA, pH 8.0)
Storage Condition 2-8 °C for short term, -20 or -80°C for long term; avoid freeze / thaw cycle
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